Features of the Immune Response to Helicobacter рylori Infection in Children with Chronic Gastroduodenal Pathology
Introduction. The immune response of a macroorganism is a determining factor for clinical manifestations of H.pylori pathogenic properties. Objective: to study the immunological features of H.pylori associated gastroduodenal pathology in children. Materials and methods. One hundred and six children aged 7 to 18 years old who were hospitalized in the Chernivtsi Regional Clinical Hospital were investigated. The presence of H.pylori was verified by histological and serological methods. The indices of CD3+, CD4+, CD8+, B cells (CD19+), T-active lymphocytes, phagocytic activity, the level of CIC, IgA, IgG, IgM, IgE, secretory IgA were determined. Statistical analysis was performed using the software Microsoft Excel 97 and Statgraphics. Results. The patients were divided into groups according to positive (72 children, 52.8 %) or negative (34 children, 32.1 %) H.pylori test and peculiarities of endoscopic changes of the stomach and duodenum structure: in 24 cases anantral superficial gastritis was diagnosed, in 22 — common superficial gastritis, in 23 children — nodular superficial gastritis, erosive gastritis in 20 and duodenal ulcer in 17 cases. Serum IgG-AT were often found both in patients and healthy individuals, IgA-AT — in children with gastroduodenal diseases. In the patients with H.pylori (+) CD3 (47.9 ± 0.8 %) and CD4 (38.8 ± 0.6 %) indices decreased. The amount of CD8 in all patients slightly increased, while H.pylori (+) significantly increased (18.5 ± 0.9 %), p < 0.05. The ratio of CD4/CD8 was significantly reduced in the patients with H.pylori (+) (2.09 ± 0.10), versus the comparative group of children (2.71 ± 0.2), p < 0.01 and in H.pylori (–) patients (2.38 ± 0.1), p < 0.05. Indices of phagocytic capacity and CIC in children with gastroduodenal pathology were low, and IgA level was increased. The most significant changes of both humoral and cellular components of immune system were observed in the patients with duodenal ulcer. Conclusion. Helicobacter pylori associated gastroduodenal diseases in children involve violation of protective mechanisms — cellular, humoral and phagocytic components of the immune system, which is the indication for immunocorrecting therapy.
Full Text:PDF (Українська)
Kornyenko EA. Infektsiya Helisobaster pylori u detey: rukovodstvo [Helicobacter pylori infection in children: a guide]. M.:HЭOTAR-Medya, 2011:272 p. (In Russian)
Shcherbak VA. Organization of therapeutic and preventive care for children with diseases of the digestive system in the Trans-Baikal Territory. Rossyyskyy vestnyk perynatolohyy y pedyatryy, 2014;59(3):99–103. (In Russian)
Sánchez Cuén JA, Irineo Cabrales AB, Bernal Magaña G, Peraza Garay F. Regression of gastric intestinal metaplasia after the eradication of Helicobacter pylori infection in a hospital in Mexico. Rev. Esp. Enferm. Dig., 2016;2:108. doi: 10.17235/reed.2016.4194/2016.
Tomb JF, White O, Kerlavage AR, et al. Stomach bacteria switch off human immune defences to cause disease. Nature, 2007;388:539-547.
Sustmann A, Okuda M, Koletzko S. Helicobacter pylori in children. Helicobacter, 2016;21(1):49-54. doi: 10.1111/hel.12341.
Shcherbak VA. New data on the etiology and pathogenesis of chronic gastroduodenitis in children [Internet]. Zabaykal'skyy medytsynskyy vestnyk, 2014;3:148–155. Avaible from: http://medacadem.chita.ru/zmv. Accessed: 11.11.2016. (In Russian)
Aksenova TA. Application Ventramin in children with peptic ulcer disease and erosive gastroduodenitis. In: Conference on Ecology and Health of Russian children. 2010; Smolensk, Russian Federation. Smolensk, 2010. (In Russian)
Shcherbak VA, Vytkovskyy YuA, Kuznyk BY. Immune disorders and their correction study in chronic gastroduodenitis in children. Medytsynskaya ymmunolohyya, 2008; 10(1):59-66. (In Russian)
Krenska-Wiacek A. Flow cytometric analysis of peripheral blood lymphocytes in children with chronic gastroduodenitis with and without Helicobacter pylori infection. Pol. Merkuriusz Lek, 2012;74(13):107–110.
Zhuravleva LN. Clinical and immunological parameters in children with H. Pylori- associated gastroduodenal diseases. Ymmunopatolohyya, allerholohyya, ynfektolohyya, 2004;4:42–47. (In Russian)
Tsymmerman YaS. Gastroduodenal disease and Helicobacter pylori-infection: a general overview of the problem. Klynycheskaya medytsyna, 2009;87(5):9-15. (In Russian)
Dvoyashkyna YuY. Local immunity and inflammatory changes vslizistoy membrane of the stomach with ulcers, associated with Helicobacter pylori. Problemy medychnoyi nauky ty osvity, 2003;4:76-79. (In Russian)
Kononov AV. The local immune response to infection Helicobacter pylori. In 12th International Symposium "Modern physiology and pathology of digestive problems", 2015, Moskow, Russian Federation. Moskow, 2015. (In Russian)
Kanareytseva TD, Chernutskaya SP, Hervazyeva VB, Sukhareva HV. Morfoimmunological ncriteria for the diagnosis of gastritis associated with Helicobacter pylori. Эksper. Klyn. Hastroэnterolohyya,2010;2:22-26. (In Russian)
Shchehlov VA. Condition immunity in chronic gastritis in children. Uspekhy sovremennoho estestvoznanyya, 2015;3:93-97. (In Russian)
Harris PR, Wright SW, Serrano C, et al. Helicobacter pylori gastritis in children is associated with a regulatory T-cell response. Gastroenterology, 2008; 134(2):491-499. doi:http://dx.doi.org/10.1053/j.gastro.2007.11.006
Shkytyn VA, Shpyrna AY, Starovoytov HN. The role of Helicobacter pylori in human pathology. Klynycheskaya mykrobyolohyya,2015;4(2):128-145. (In Russian)
Pachathundikandi SK, Müller A, Backert S. Inflammasome activation by Helicobacter pylori and its Implications for Persistence and Immunity. Curr. Top. Microbiol. Immunol., 2016; 397:117-31. doi: 10.1007/978-3-319-41171-2-6.
- There are currently no refbacks.
Copyright (c) 2017 ACTUAL INFECTOLOGY
This work is licensed under a Creative Commons Attribution 4.0 International License.
© Publishing House Zaslavsky, 1997-2018